Physiology
The body needs to keep blood pH in a very tight range; typically between 7.35 and 7.45. Outside of this range, bad things can happen; Abnormalities in:
Electrolyte concentrations - esp calcium and potassium
Protein folding
Membrane potentials
Cellular function
May seem like a narrow range - remember that pH is a logarithmic scale. Small changes in pH mean much larger changes in serum proton (H+) concentration
We are constantly exposed to substances or changes in metabolism that, if left uncompensated, would result in large changes in pH. Our body is able to keep pH in such a narrow range through 3 main mechanisms:
Bicarbonate buffer equation - Resist changes in pH through LeChatlier’s principle
The lungs - Hyper or hypo-ventilate
The kidneys
Secrete or retain acid
Secrete or retain bicarbonate
Produce de novo bicarbonate
One honorable mention - hemoglobin, acts as a potent buffer by binding carbon dioxide to form carbaminohemoglobin
History of ABG interpretation
Van Slyke - popularized a method using Bronsted-Lowry definition of Acids/bases and the Henderson-Hassalbach equation
Bronsted-Lowry define acids as something that donates proton in solution and bases as something that accepts proton in solution
Henderson hassalbach equation relates pH, pCO2, Bicarbonate.
Donald Van Slyke was a Dutch American Biochemist who popularized the method of acid-base interpretation that most of us use to this day all the way back in the 1950s
His methods at the time were only able to measure blood pCO2 and blood pH. Serum bicarbonate was calculated from this values
Most of the van slyke method involved interpreting patterns that arose from abnormalities in pH, bicarbonate, and pCO2 alone
There is a different, more quantitative approach to acid-base that takes a lot more things into consideration, like the effects of chloride, lactate, and the strong-ion difference on acid-base status
This called the Stewart approach, popularized by Peter Stewart back in the 1980s. Stewart used about 6 equations inspired by many different fields of analytical chemistry to interpret physiologic acid-base status.
More resources:
pH <7.35 - Acid
pH >7.45 - Base
What makes sense?
Carbon dioxide = Acid
High acid (CO2) should give you respiratory acidosis, low acid should give you respiratory alkalosis
Bicarb = base
High base (bicarb) should give you metabolic alkalosis, low base should give you metabolic acidosis
Attributions
coming soon